Host cell protein dynamics in recombinant CHO cells
نویسندگان
چکیده
منابع مشابه
Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CHO Cells
Chinese hamster ovary (CHO) cells are the most common cell line used for the production of therapeutic proteins including monoclonal antibodies (mAbs). Host cell proteins (HCPs), secreted and released from lysed cells, accumulate extracellularly during the cultures of recombinant CHO (rCHO) cells, potentially impairing product quality. In an effort to maintain good mAb quality during the cultur...
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Background Productivity of recombinant therapeutics is a coordinated effort of multiple pathways in the cell [1]. The protein processing pathway in endoplasmic reticulum has been the target of many cell engineering studies but with mixed results [2]. We have observed the induction of UPR genes in recombinant CHO cells (data not shown). In this work, we attempt to increase their productivity fur...
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Therapeutic glycoproteins have played a major role in the commercial success of biotechnology in the post-genomic era. But isolating recombinant mammalian cell lines for large-scale production remains costly and time-consuming, due to substantial variation and unpredictable stability of expression amongst transfected cells, requiring extensive clone screening to identify suitable high producers...
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Recombinant protein therapeutics have changed the face of modern medicine in the past decade, and they continue to provide innovative and effective therapies for numerous previously refractory illnesses. Today, they are used in the treatment of a variety of human diseases, ranging from cancers to infertility. These proteins are generally synthesized by large-scale cultivation of genetically eng...
متن کاملCloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells
Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected ...
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ژورنال
عنوان ژورنال: Bioengineered
سال: 2013
ISSN: 2165-5979,2165-5987
DOI: 10.4161/bioe.23382